Nadia Commanechi, Sri (2025) FUNGI ENDOFIT SEBAGAI AGEN BIOKONTROL PATOGEN UTAMA TANAMAN CABAI MERAH : UJI ANTAGONISME DAN MEKANISME SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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Abstract
Endophytic fungi, particularly dark septate endophytes (DSE), have emerged as a promising and sustainable strategy for mitigating fungal pathogens in chili plants. Effective diseases management is critical in chili production, as fungal infections can cause significant crop losses, reduce fruit quality, and decrease yield. While the use of endophytic fungi in plant protection has been explored in various crops, the potential of DSE as a biocontrol against fungal pathogens in chili plants remains unknown. The present study aimed to evaluate the effectiveness of DSE fungi as biological antagonists against major pathogens that cause destructive diseases in chili plants, using in vitro trials. The study was conducted from December 2023 to March 2024 at the National Research and Innovation Agency (BRIN). A completely randomized design (CRD) was employed, with a single factor—DSE treatment. Three treatments included D0 (control), DSE 411, and DSE 471. Four pathogenic species were tested: Fusarium oxysporum (Fo), Phytophthora capsici (Py), Colletotrichum capsici (Cc), and Alternaria sp. (Alt). Inhibition rates (IR) were analyzed using Analysis of Variance (ANOVA), followed by the Least Significant Difference (LSD) test at a 5% significance level. The highest antagonistic activity was observed against C. capsici (Cc), with DSE 411 showing a mean value of IR of 77.54%, whereas DSE 471 exhibited a significantly higher IR of 90.26%. Macroscopic observations revealed the formation of clear zones around both DSE colonies, suggesting the mechanism of inhibition by antibiosis or the production of antifungal compounds. Microscopic analysis using scanning electron microscopy (SEM) revealed specific antagonistic mechanisms, including parasitism and competition, as key factors in pathogen suppression. These findings highlight the potential use of DSE fungi as effective biocontrol agents for managing major diseases in chili plants, offering a promising strategy for sustainable disease management and crop protection.
| Item Type: | Thesis (S1) | ||||||||||||
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| Additional Information: | Cendawan endofit, khususnya dark septate endophytes (DSE), telah muncul sebagai strategi yang menjanjikan dan berkelanjutan untuk memitigasi patogen cendawan pada tanaman cabai. Pengelolaan patogen yang efektif sangat penting dalam produksi cabai, karena infeksi cendawan dapat menyebabkan kehilangan hasil panen yang signifikan, menurunkan kualitas buah, dan menurunkan hasil panen. Meskipun penggunaan cendawan endofit dalam perlindungan tanaman telah dieksplorasi pada berbagai tanaman, potensi DSE sebagai biokontrol terhadap patogen cendawan pada tanaman cabai masih belum diketahui. Penelitian ini bertujuan untuk mengevaluasi keefektifan cendawan DSE sebagai antagonis hayati terhadap patogen utama penyebab penyakit yang merusak pada tanaman cabai, dengan menggunakan uji in vitro. Penelitian ini dilakukan dari Desember 2023 hingga Maret 2024 di Badan Riset dan Inovasi Nasional (BRIN). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan perlakuan faktor tunggal-DSE. Tiga perlakuan yang diuji adalah D0 (kontrol), DSE 411, dan DSE 471. Empat spesies patogen diuji: Fusarium oxysporum (Fo), Phytophthora capsici (Py), Colletotrichum capsici (Cc), dan Alternaria sp. Tingkat penghambatan (IR) dianalisis menggunakan Analysis of Variance (ANOVA), diikutI Rancangan Acak Lengkap (RAL) pada tingkat signifikansi 5%. Aktivitas antagonis tertinggi diamati terhadap C. capsici (Cc), dengan DSE 411 menunjukkan nilai rata-rata 77,54%, sedangkan DSE 471 menunjukkan nilai rata-rata yang jauh lebih tinggi yaitu 90,26%. Pengamatan makroskopis menunjukkan terbentuknya zona bening di sekitar kedua koloni DSE, yang menunjukkan mekanisme penghambatan oleh antibiosis atau produksi senyawa antifungi atau anti cendawan. Analisis mikroskopis menggunakan scanning electron microscopy (SEM) mengungkapkan mekanisme antagonis spesifik, termasuk parasitisme dan kompetisi, sebagai faktor kunci dalam penekanan patogen. Temuan ini menyoroti potensi penggunaan cendawan DSE sebagai agen biokontrol yang efektif untuk mengelola penyakit utama pada tanaman cabai, menawarkan strategi yang menjanjikan untuk pengelolaan penyakit dan perlindungan tanaman yang berkelanjutan. | ||||||||||||
| Uncontrolled Keywords: | biocontrol, dark septate endophytes (DSE), inhibition rate, parasitism biocontrol, dark septate endophytes (DSE), laju penghambatan, parasitisme | ||||||||||||
| Subjects: | S Agriculture > SB Plant culture | ||||||||||||
| Divisions: | 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi 04-Fakultas Pertanian |
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| Depositing User: | Mrs Sri Nadia Commanechi | ||||||||||||
| Date Deposited: | 13 Mar 2025 04:53 | ||||||||||||
| Last Modified: | 13 Mar 2025 04:53 | ||||||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/46095 |
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