RACHMAWATI, IKE (2024) IDENTIFIKASI MORFOLOGI DAN KERAGAMAN GENETIK TANAMAN SAWO(Manilkara zapota (L.) van Royen) di KOTA CILEGON DAN KOTA SERANG PROVINSI BANTEN. Master thesis, PASCASARJANA.
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Abstract
The sapodilla genetic diversity in Banten Province has not been utilized optimally. Limited information regarding superior varieties of sapodilla cultivars, lack of exploration efforts and extracting genetic information on potential germplasm of sapodilla plants are obstacles in its development. The aim of the research was to determine the diversity of local sapodilla germplasm in Cilegon City and Serang City based on morphological characters, genetic characters, identifying characters and to determine the relationship between morphological characters and genetic characters. This research uses a descriptive method with a quantitative approach. Morphological characterization research was carried out in Cikerai Village, Cilegon City and in Cikulur Village, Serang City, Banten Province using purposive sampling techniques. Genetic characterization research was carried out at the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sultan Ageng Tirtayasa University from August to October 2023. Cluster analysis was used to determine the distance or closeness of the relationship between individual plants which can be seen in the form of a dendogram and to determine the characteristic characters, PCA analysis was used. (Principal Component Analysis). The results of the research show that there is a diversity of morphological and genetic characters in the sapodilla plant germplasm in Cilegon City and Serang City. The morphological characteristic is the shape of the curve of the leaf tip. The genetic marker that is capable of identifying each accession is the Sap SSR_15 marker and the marker based on a combined analysis of morphological and genetic characters is the shape of the leaf tip curve.
| Item Type: | Thesis (Master) | |||||||||
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| Additional Information: | Keanekaragaman genetik sawo di Provinsi Banten belum dimanfaatkan secara optimal. Terbatasnya informasi mengenai kultivar sawo varietas unggul, kurangnya usaha eksplorasi dan penggalian informasi genetik potensi plasma nutfah tanaman sawo menjadi kendala dalam pengembangannya. Tujuan penelitian untuk mengetahui keragaman plasma nutfah sawo lokal di Kota Cilegon dan Kota Serang berdasarkan karakter morfologi, karakter genetik, karakter penciri serta mengetahui hubungan antara karakter morfologi dan karakter genetik. Penelitian ini menggunakan metode deskriptif dengan pendekatan kuantitatif. Penelitian karakterisasi morfologi dilaksanakan di Desa Cikerai Kota Cilegon dan di Desa Cikulur Kota Serang Provinsi Banten menggunakan teknik purposive sampling. Penelitian karakterisasi genetik dilaksanakan di Laboratorium Fisiologi dan Bioteknologi Tanaman Fakultas Pertanian Universitas Sultan Ageng Tirtayasa pada bulan Agustus sampai dengan Oktober 2023. Analisis Cluster digunakan untuk menentukan jauh atau dekatnya hubungan kekerabatan antar individu tanaman yang terlihat dalam bentuk dendogram dan untuk menentukan karakter penciri digunakan Analisis PCA (Principal Component Analisys). Hasil penelitian menunjukan terdapat keragaman karakter morfologi dan genetik pada plasma nutfah tanaman sawo di Kota Cilegon dan Kota Serang. Penciri secara morfologi adalah Bentuk Lekuk Ujung Daun. Penciri secara genetik marka molekuler yang mampu mengidentifikasi setiap aksesi adalah marka Sap SSR_15 dan penciri berdasarkan analisis gabungan karakter morfologi dan genetik adalah Bentuk Lekuk Ujung Daun. | |||||||||
| Uncontrolled Keywords: | Genetic Diversity, Manilkara zapota (L.) van Royen, Plasma- Nutfah, SSR Keragaman Genetik, Manilkara zapota (L.) van Royen, Plasma- Nutfah, SSR | |||||||||
| Subjects: | S Agriculture > S Agriculture (General) | |||||||||
| Divisions: | 08-Pascasarjana > 54108-Magister Ilmu Pertanian 08-Pascasarjana |
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| Depositing User: | Ike Rachmawati | |||||||||
| Date Deposited: | 25 Sep 2024 16:22 | |||||||||
| Last Modified: | 25 Sep 2024 16:22 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/42453 |
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