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POTENSI AKTINOMISETES ASAL RIZOSFER PISANG BILE SEBAGAI AGEN BIOKONTROL PENYAKIT LAYU FUSARIUM PISANG (Rekomendasi Konten Pembelajaran Biologi SMA)
Indonesia, as one of the world's largest banana producers, faces a serious threat from Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4 (TR4), a pathogen that is difficult to control with fungicides due to the presence of chlamydospores that persist in the soil. This study aims to investigate the potential of actinomycetes from the rhizosphere of Bile bananas as biocontrol agents for Fusarium wilt disease and to recommend the results of the study as content for high school biology education. The methods used included in vitro screening of Bile banana rhizosphere isolates against Foc TR4 C-S4.1, characterization of biocontrol activity and molecular identification of biocontrol agent actinomycetes, and preparation of recommendations for high school biology learning content. The results showed that six isolates with potential as biocontrol agents were obtained, namely B1-1.6, B1-1.11, B2-1.9, B2-1.21, B3-1.2, and B3-1.3, with the highest inhibition index of 70.2% from isolate B2-1.9. Isolates B1-1.6, B1-1.11, B3-1.2, and B3-1.3 showed a biocontrol mechanism in the form of protease, chitinase, and siderophore enzyme production. The identification results showed that these isolates belonged to the genus Streptomyces, including S. racemochromogenes (B1-1.6, B3-1.2, and B3-1.3), S. chattanoogensis (B1-1.11), and S. abikoensis (B2-1.9 and B2-1.21). These findings indicate a correlation between activity and antagonism and a biocontrol mechanism that confirms the potential. These findings indicate a correlation between activity and antagonism and a biocontrol mechanism that confirms the potential of Actinomycetes in the rhizosphere of Bile bananas as a biocontrol agent for the sustainable control of Fusarium wilt disease. The results of this study are recommended as high school biology learning content for material on the diversity of living things, their interactions, and their roles in nature (Phase E), the growth and development of living things (Phase F), and biotechnology innovations (Phase F).
PENDIDIKAN BIOLOGI, UNIVERSITAS SULTAN AGENG TIRTAYASA
POTENSI AKTINOMISETES ASAL RIZOSFER PISANG BILE SEBAGAI AGEN BIOKONTROL PENYAKIT LAYU FUSARIUM PISANG (Rekomendasi Konten Pembelajaran Biologi SMA) (Text)
POTENSI AKTINOMISETES ASAL RIZOSFER PISANG BILE SEBAGAI AGEN BIOKONTROL PENYAKIT LAYU FUSARIUM PISANG (Rekomendasi Konten Pembelajaran Biologi SMA) (Other)
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L Education (General)
Q Science (General)
QR Microbiology
SB Plant culture