relation: https://eprints.untirta.ac.id/49093/
title: Eksplorasi dan Uji Antagonisme Cendawan Endofit Asal Alang-alang (Imperata cylindrica L. Beauv.) dalam Mengendalikan Fusarium oxysporum f.sp. cubense Pada Tanaman Pisang secara In Vitro
creator: Muthiah, Siti
subject: S Agriculture (General)
description: The pathogenic fungi Fusarium oxysporum f.sp. cubense (Foc) can damage banana plants by causing fusarium wilt disease. In biological control efforts, endophytic fungi can be applied as biological agents to suppress pathogen growth. Endophytic fungi have antagonistic potential in inhibiting the growth of their host. This study aimed to isolate endophytic fungi from the roots of cogon grass (Imperata cylindrica L. Beauv.) and analyze the antagonistic ability of endophytic fungi in inhibiting the growth of the pathogenic fungus Foc in vitro. This research was a quantitative research with exploration and experimental methods. This study used a non-factorial Completely Randomized Design with five levels of treatment in five replicates. The treatments were: cogon grass endophytic fungus 1 (CEA1), cogon grass endophytic fungus 2 (CEA2), cogon grass endophytic fungus 3 (CEA3), positive control (CP0), and negative control (CPF0). The observed variables were: percentage inhibition, area of fungal colony growth, macroscopic morphological characterization, and pathogenicity. The results showed that 23 isolates of endophytic fungi were successfully isolated from roots of cogon grass, of which six had antagonistic activity. Three isolates with strong antagonism activity were encoded CEA1, CEA2, and CEA3. Based on the antagonism test with the dual culture method, the three isolates were antagonistic to the Foc fungus with varying inhibition. Isolate CEA3 showed the best results in inhibiting Foc growth at 4-7 Days After Inoculation (HSI) with a percentage inhibition of up to 58.21% and suppressed the growth area of Foc colonies up to 4.74 cm. The macroscopic characteristics of CEA3 has irregular colony shape, flat topography, granular surface texture, greenish white color, and exudate droplets. Pathogenicity test showed that the isolate CEA3 was not pathogenic.
date: 2025
type: Thesis
type: NonPeerReviewed
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/1/Siti%20Muthiah_4442210123_Fulltext.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/3/Siti%20Muthiah_4442210123_01.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/4/Siti%20Muthiah_4442210123_02.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/5/Siti%20Muthiah_4442210123_03.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/6/Siti%20Muthiah_4442210123_04.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/7/Siti%20Muthiah_4442210123_05.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/8/Siti%20Muthiah_4442210123_Ref.pdf
format: text
language: id
identifier: https://eprints.untirta.ac.id/49093/9/Siti%20Muthiah_4442210123_Lamp.pdf
identifier:   Muthiah, Siti  (2025) Eksplorasi dan Uji Antagonisme Cendawan Endofit Asal Alang-alang (Imperata cylindrica L. Beauv.) dalam Mengendalikan Fusarium oxysporum f.sp. cubense Pada Tanaman Pisang secara In Vitro.  S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.