MA’RIFAT, MILA (2018) INDUKSI DAN MULTIPLIKASI KALUS DARI JARINGAN ENDOSPERMA SIRSAK (Annona muricata L.) SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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INDUKSI DAN MULTIPLIKASI KALUS DARI JARINGAN ENDOSPERMA SIRSAK (Annona muricata L.) SECARA IN VITRO.PDF Restricted to Registered users only Download (3MB) |
Abstract
Endosperm tissue culture is one of method to produce triploid plants. In the process, the application of growth regulating substances was needed in the success of tissue culture. This research aims to determine the concentrations of 2,4-D growth regulators, tomato extract and kinetin which was appropriate for the induction and multiplication of callus. Also, this research was conducted in the Biotechnology Laboratory of Agriculture Faculty, Sultan Ageng Tirtayasa University, Banten. From February to June 2018 and used complete random design and repeated three times with two factors and there are two stages, was callus induction and subculture (multiplication). As for some concentrations of growth regulators used in the callus induction stage of 0 ppm (control), 1 ppm, 2 ppm, 3 ppm and 4 ppm for growth regulators 2,4-D and 0 g/l (control), 100 g/l and 150 g/l for tomato extract. While for the subculture stage (multiplication) concentrations of 2,4-D growth regulators used were 1 ppm, 2 ppm, 3 ppm and 4 ppm and concentrations of kinetin growth regulators was 0.5 ppm, 1 ppm and 1,5 ppm. The Research of soursop endosperm culture obtained the best concentration of growth regulators in the application of 2,4-D growth regulators with a concentration of 2 ppm which significantly affected the diameter of soursop endosperm explants at callus induction stage with an average diameter of 5.27 cm. The concentration of 2 ppm and 3 ppm was also estimated for influential the scoring of soursop endosperm explants by the scoring was two. While, the subculture stage (multiplication) the best concentration of growth regulators was 3 ppm of 2,4-D which combined with 1,5 ppm kinetin had an effect on callus formation scoring and the scoring was three. However, for the using of tomato extract, the results were very significant for the explant browning percentage with an average percentage of 0% while for explants grown without tomato extract obtained the highest percentage of 100%.
| Item Type: | Thesis (S1) | |||||||||
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| Additional Information: | Tingginya permintaan pasar terhadap buah sirsak menjadikan prospek pembudidayaan buah sirsak sangat berpeluang tinggi dibidang ekonomi selain dimanfatkan sebagai buah segar, sudah banyak produk-produk minuman maupun makanan yang berbahan dasar sirsak dan menjadikan buah ini sebagai buah unggulan indonesia. Akan tetapi karena jumlah bijinya yang sangat banyak sehingga menyebabkan menurunnya tingkat kepuasan konsumen. Oleh sebab itu perbaikan plasma nuftah sangatlah diperlukan guna meminimalisir permasalahan tersebut. Salah satu teknologi inovasi yang dilakukan yaitu dengan cara menghasilkan tanaman sirsak triploid atau buah sirsak tanpa biji. Dengan cara mengkulturkan endosperma buah sirsak tersebut. Pengaplikasian zat pengatur tumbuh pada kultur endosperma merupakan salah satu hal yang pokok dan menjadi parameter keberhasilan dalam proses kultur in vitro, sehingga penelitian ini bertujuan untuk mengetahui konsentrasi zat pengatur tumbuh 2,4-D , ekstrak tomat dan kinetin yang tepat untuk induksi dan multiplikasi kalus. Penelitian ini telah dilakukan dilaboratorium Bioteknologi Jurusan Agroekoteknologi Fakultas Pertanian Universitas Sultan Ageng Tirtayasa SerangBanten. Terdapat dua tahapan yang dilakukan yaitu tahapan induksi kalus dan tahapan subkultur (multiplikasi). Adapun beberapa konsentrasi zat pengatur tumbuh yang digunakan pada tahap induksi kalus yaitu 0 ppm ( kontrol), 1 ppm, 2 ppm, 3 ppm dan 4 ppm untuk ZPT 2,4-D dan 0 g/l ( Kontrol), 100 g/l dan 150 g/l untuk ekstrak tomat, sedangkan untuk tahapan subkultur (multiplikasi) konsentrasi 2,4-D yang digunakan yaitu 1 ppm, 2 ppm, 3 ppm dan 4 ppm dan konsentrasi 0,5 ppm, 1 ppm dan 1,5 ppm pada kinetin. | |||||||||
| Subjects: | S Agriculture > SB Plant culture | |||||||||
| Divisions: | 04-Fakultas Pertanian 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi |
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| Depositing User: | Admin Eprints Untirta | |||||||||
| Date Deposited: | 08 Dec 2021 10:29 | |||||||||
| Last Modified: | 08 Dec 2021 10:29 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/9526 |
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