ASTUTI, ANISA DWI (2025) POTENSI AKTINOMISETES ASAL RIZOSFER PISANG BILE SEBAGAI AGEN BIOKONTROL PENYAKIT LAYU FUSARIUM PISANG (Rekomendasi Konten Pembelajaran Biologi SMA). S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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Abstract
Indonesia, as one of the world's largest banana producers, faces a serious threat from Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4 (TR4), a pathogen that is difficult to control with fungicides due to the presence of chlamydospores that persist in the soil. This study aims to investigate the potential of actinomycetes from the rhizosphere of Bile bananas as biocontrol agents for Fusarium wilt disease and to recommend the results of the study as content for high school biology education. The methods used included in vitro screening of Bile banana rhizosphere isolates against Foc TR4 C-S4.1, characterization of biocontrol activity and molecular identification of biocontrol agent actinomycetes, and preparation of recommendations for high school biology learning content. The results showed that six isolates with potential as biocontrol agents were obtained, namely B1-1.6, B1-1.11, B2-1.9, B2-1.21, B3-1.2, and B3-1.3, with the highest inhibition index of 70.2% from isolate B2-1.9. Isolates B1-1.6, B1-1.11, B3-1.2, and B3-1.3 showed a biocontrol mechanism in the form of protease, chitinase, and siderophore enzyme production. The identification results showed that these isolates belonged to the genus Streptomyces, including S. racemochromogenes (B1-1.6, B3-1.2, and B3-1.3), S. chattanoogensis (B1-1.11), and S. abikoensis (B2-1.9 and B2-1.21). These findings indicate a correlation between activity and antagonism and a biocontrol mechanism that confirms the potential. These findings indicate a correlation between activity and antagonism and a biocontrol mechanism that confirms the potential of Actinomycetes in the rhizosphere of Bile bananas as a biocontrol agent for the sustainable control of Fusarium wilt disease. The results of this study are recommended as high school biology learning content for material on the diversity of living things, their interactions, and their roles in nature (Phase E), the growth and development of living things (Phase F), and biotechnology innovations (Phase F).
| Item Type: | Thesis (S1) | |||||||||||||||
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| Additional Information: | Indonesia sebagai salah satu produsen pisang terbesar di dunia menghadapi ancaman serius dari penyakit Layu Fusarium pisang yang disebabkan oleh Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4 (TR4), patogen yang sulit dikendalikan dengan fungisida karena keberadaan klamidospora yang bertahan lama di tanah. Penelitian ini bertujuan mempelajari potensi aktinomisetes asal rizosfer pisang Bile sebagai agen biokontrol penyakit Layu Fusarium Pisang serta merekomendasikan hasil penelitian sebagai konten pembelajaran Biologi SMA. Metode yang digunakan meliputi skrining isolat rizosfer pisang Bile secara in vitro terhadap Foc TR4 C-S4.1, karakterisasi aktivitas biokontrol dan identifikasi molekuler aktinomisetes agen biokontrol, serta penyusunan rekomendasi konten pembelajaran Biologi SMA. Hasil penelitian menunjukkan bahwa enam isolat yang berpotensi sebagai agen biokontrol berhasil diperoleh, yaitu B1-1.6, B1-1.11, B2-1.9, B2-1.21, B3-1.2, dan B3-1.3, dengan indeks penghambatan tertinggi sebesar 70,2% dari isolat B2-1.9. Isolat B1-1.6, B1-1.11, B3-1.2, dan B3-1.3 menunjukkan mekanisme biokontrol berupa produksi enzim protease, kitinase, dan siderofor. Hasil identifikasi menunjukkan bahwa isolat-isolat tersebut merupakan genus Streptomyces, meliputi S. racemochromogenes (B1-1.6, B3-1.2, dan B3-1.3), S. chattanoogensis (B1-1.11), dan S. abikoensis (B2-1.9, dan B2-1.21). Temuan ini menunjukkan korelasi aktivitas dan antagonisme dan mekanisme biokontrol yang menegaskan potensi Aktinomisetes rizosfer pisang Bile sebagai agen biokontrol untuk pengendalian penyakit layu Fusarium yang berkelanjutan. Hasil penelitian ini direkomendasikan sebagai konten pembelajaran Biologi SMA untuk materi keanekaragaman makhluk hidup, interaksi, dan peranannya di alam (Fase E), pertumbuhan dan perkembangan makhluk hidup (Fase F), serta inovasi bioteknologi (Fase F). | |||||||||||||||
| Subjects: | L Education > L Education (General) Q Science > Q Science (General) Q Science > QR Microbiology S Agriculture > SB Plant culture |
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| Divisions: | 02-Fakultas Keguruan dan Ilmu Pendidikan 02-Fakultas Keguruan dan Ilmu Pendidikan > 84205-Jurusan Pendidikan Biologi |
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| Depositing User: | Anisa Dwi Astuti | |||||||||||||||
| Date Deposited: | 12 Feb 2026 02:04 | |||||||||||||||
| Last Modified: | 12 Feb 2026 02:04 | |||||||||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/58193 |
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