Fitriana, Tarishah (2025) PENGARUH JENIS NUTRIENT NITROGEN PADA PEMANFAATAN LIMBAH CAIR PATI TALAS BENENG SEBAGAI SUBSTRAT FERMENTASI SELULOSA BAKTERI. S1 thesis, Fakultas Teknik Universitas Sultan Ageng Tirtayasa.
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Abstract
The tuber-based food processing industry in Indonesia continues to grow, including the Beneng taro (Xanthosoma undipes) whose production reached more than 20 thousand tons in 2022. The Beneng taro starch processing process produces large amounts of liquid waste that has the potential to be used as a fermentation substrate to produce bacterial cellulose (BC). Utilization of this liquid waste not only offers solutions to environmental problems but also increases added value. The uniqueness of this research lies in the utilization of Beneng taro starch liquid waste as an alternative fermentation medium that has not been widely explored, the use of bean sprout extract as a natural nitrogen source to replace inorganic nitrogen, and a combinative approach between optimizing glucose levels and nitrogen types. This study aims to analyze the effect of variations in glucose concentration (5–20%) and types of nitrogen sources (urea, ZA, and bean sprout extract) on BC characteristics including pH, wet weight, thickness, yield, water content, cellulose content, and swelling capacity. The research method includes making bean sprout extract by grinding bean sprouts, filtering and taking the extract as a natural nitrogen source. Furthermore, bacterial cellulose was made by utilizing liquid waste of taro starch as a fermentation medium, which was added with glucose according to the selected variation and nitrogen source, then inoculated with Acetobacter xylinum and fermented statically for 14 days at room temperature. The results showed a trend of increasing BC characteristics along with increasing glucose concentration. The best results were obtained at a variation of 20% glucose with organic nitrogen from bean sprout extract, characterized by a BC wet weight of 41.93 g, a thickness of 6.375 mm, a yield of 40.37%, a water content of 58.39%, a cellulose content of 41.60%, and a swelling capacity of 1.40.
| Item Type: | Thesis (S1) | ||||||
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| Additional Information: | Industri pengolahan pangan berbasis umbi-umbian di Indonesia terus berkembang, termasuk talas beneng (Xanthosoma undipes) yang produksinya mencapai lebih dari 20 ribu ton pada tahun 2022. Proses pengolahan pati talas beneng menghasilkan limbah cair dalam jumlah besar yang berpotensi dimanfaatkan sebagai substrat fermentasi untuk menghasilkan bacterial cellulose (BC). Pemanfaatan limbah cair ini tidak hanya menawarkan solusi terhadap permasalahan lingkungan, tetapi juga meningkatkan nilai tambah. Kekhasan penelitian ini terletak pada pemanfaatan limbah cair pati talas beneng sebagai media fermentasi alternatif yang belum banyak dieksplorasi, penggunaan ekstrak tauge sebagai sumber nitrogen alami pengganti nitrogen anorganik, serta pendekatan kombinatif antara optimasi kadar glukosa dan jenis nitrogen. Penelitian ini bertujuan untuk menganalisis pengaruh variasi konsentrasi glukosa (5–20%) dan jenis sumber nitrogen (urea, ZA, dan ekstrak tauge) terhadap karakteristik BC meliputi pH, berat basah, ketebalan, rendemen, kadar air, kadar selulosa, dan kapasitas swelling. Metode penelitian meliputi pembuatan ekstrak tauge dengan penghalusan tauge, menyaring dan mengambil ekstraknya sebagai sumber nitrogen alami, Selanjutnya dilakukan pembuatan bacterial cellulose dengan memanfaatkan limbah cair pati talas beneng sebagai media fermentasi, yang ditambahkan glukosa sesuai variasi dan sumber nitrogen terpilih, kemudian diinokulasi dengan Acetobacter xylinum dan difermentasi secara statis selama 14 hari pada suhu ruang. Hasil penelitian menunjukkan adanya tren peningkatan karakteristik BC seiring dengan bertambahnya konsentrasi glukosa. Hasil terbaik diperoleh pada variasi 20% glukosa dengan nitrogen organik dari ekstrak tauge, ditandai dengan berat basah BC sebesar 41,93 g, ketebalan 6,375 mm, rendemen 40,37%, kadar air 58,39%, kadar selulosa 41,60%, serta kapasitas swelling 1,40. | ||||||
| Subjects: | Q Science > QR Microbiology | ||||||
| Divisions: | 03-Fakultas Teknik 03-Fakultas Teknik > 24201-Jurusan Teknik Kimia |
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| Depositing User: | Tarishah Fitriana | ||||||
| Date Deposited: | 01 Dec 2025 07:03 | ||||||
| Last Modified: | 01 Dec 2025 07:03 | ||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/56383 |
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