ISMIAH, ALVI (2025) APLIKASI BAKTERI ENDOFIT ASAL PUTRI MALU (Mimosa pudica L.) UNTUK PENGENDALIAN LAYU FUSARIUM PISANG KEPOK (Musa acuminata X balbisiana) SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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Abstract
Fusarium oxysporum f. sp. cubense (Foc) is a pathogenic fungus that causes fusarium wilt disease on banana plants. The Foc Tropical Race 4 (TR4) appeared which was stronger and resistant to pesticides, banana plant cultivar could not be used as a natural enemy of Foc TR4. This research aims to detect the presence of endophytic bacteria derived from the roots of Putri Malu plants (Mimosa pudica L.), the antagonistic potential of endophytic bacteria in inhibiting the growth of fusarium will be analyzed and the macroscopic characteristics of endophyte bacteria will be described. This research uses a non-factorial Completely Randomized Design with five treatments, CP0 (negative control), CPF0 (positive control), and an endophytic bacteria isolate of Mimosa pudica L. (BEP5, BEP8, and BEP15) and four repetitions. The obtained bacteria isolate was tested in vitro with the dual culture method. The results showed that the three isolates of endophytic bacteria were able to inhibit the growth of fusarium in 1-5 Days After Inoculation, with the highest percentage of inhibition including BEP5 37,122%, BEP8 36,601%, and BEP15 37,522%. The result shows that endophytic bacteria derived from mimosa plant roots can potentially be biological control agents.
| Item Type: | Thesis (S1) | |||||||||
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| Additional Information: | Fusarium oxysporum f. sp. cubense (Foc) adalah jamur patogen yang menyebabkan penyakit layu fusarium pada tanaman pisang. Muncul Foc Tropical Race 4 (TR4) yang lebih kuat dan resisten terhadap pestisida, belum ada kultivar tanaman pisang yang dapat dijadikan musuh alami Foc TR4. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri endofit yang berasal dari akar tanaman Putri Malu (Mimosa pudica L.), potensi antagonis bakteri endofit dalam menghambat pertumbuhan fusarium akan dianalisis dan mengidentifikasi karakteristik makroskopis bakteri endofit. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non-faktorial dengan lima perlakuan, CP0 (kontrol negatif), CPF0 (kontrol positif), dan isolat bakteri endofit Mimosa pudica L. (BEP5, BEP8, dan BEP15) dengan empat pengulangan. Isolat bakteri yang diperoleh diuji secara in vitro dengan metode kultur ganda. Hasil penelitian menunjukkan bahwa ketiga isolat bakteri endofit tersebut mampu menghambat pertumbuhan fusarium dalam 1-5 Hari Setelah Inokulasi (HSI), dengan persentase penghambatan tertinggi antara lain BEP5 37,122%, BEP8 36,601%, dan BEP15 37,522%. Hasil penelitian menunjukkan bahwa bakteri endofit yang berasal dari akar tanaman mimosa berpotensi menjadi agen pengendalian biologis. | |||||||||
| Subjects: | S Agriculture > S Agriculture (General) | |||||||||
| Divisions: | 04-Fakultas Pertanian 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi |
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| Depositing User: | Mrs. Alvi Ismiah | |||||||||
| Date Deposited: | 29 Apr 2025 03:26 | |||||||||
| Last Modified: | 29 Apr 2025 03:26 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/48303 |
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