POTENSI FUNGI ENDOFIT ASAL PISANG LIAR PANDEGLANG SEBAGAI AGEN PENGENDALI HAYATI PANAMA DISEASE (Sebagai Rekomendasi Bahan Ajar Untuk Siswa SMA Kelas X Pada Materi Bioteknologi)

Zahro, Fathimah (2024) POTENSI FUNGI ENDOFIT ASAL PISANG LIAR PANDEGLANG SEBAGAI AGEN PENGENDALI HAYATI PANAMA DISEASE (Sebagai Rekomendasi Bahan Ajar Untuk Siswa SMA Kelas X Pada Materi Bioteknologi). S1 thesis, Universitas Sultan Ageng Tirtayasa.

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Abstract

Indonesia, a tropical country in Southeast Asia, ranks third globally as a banana producer after India and China. Although banana cultivation in Indonesia is quite high, the threat of disease, especially Panama Disease, is still a serious problem. Conventional approaches using fungicides have been found to be ineffective against TR4 (Tropical Race 4) chlamydiosphore. This study aims to explore endophytic fungi from wild bananas in Pandeglang as biological control agents against Panama disease. This study used in vitro, in planta methods, and observed the effects of endophytic fungi on Cavendish banana. Three selected endophytic fungus, identified as Fusarium sp. NMC278, NMC279, and NMC293, showed effective biological control potential against Panama disease, with inhibition percentages ranging from 10%-11% in vitro. The mechanism of biological control involves antibiosis through the release of secondary metabolites capable of inhibiting the pathogen, showing great potential against Panama disease. In planta tests showed induced growth in banana plants, reflected in increased stem diameter and number of roots. Detection of phenolic compounds showed that the inoculated endophytic fungi contributed to plant resistance, with positive reactions to glycosides, tannins and saponins. The educational implications of this study recommend the development of a digital encyclopedia (59.7%) as teaching material, based on a needs analysis of teachers and students in Senior High School (SMA).

Item Type:

Thesis (S1)

Contributors:

Contribution	Contributors	NIP/NIM
Thesis advisor	Maryani, Nani	198307292008122001
Thesis advisor	Oktorida Khastini, Rida	198110282008012017

Additional Information:

Indonesia sebagai negara tropis di Asia Tenggara, menempati peringkat ke-3 secara global sebagai produsen pisang setelah India dan China. Meskipun budidaya pisang di Indonesia cukup tinggi, namun ancaman penyakit khususnya penyakit Panama Disease, masih menjadi masalah serius. Pendekatan konvensional dengan menggunakan fungisida ternyata tidak efektif melawan klamidiosfor TR4 (Tropical Race 4). Penelitian ini bertujuan untuk mengeksplorasi fungi endofit dari pisang liar di Pandeglang sebagai agen pengendali biologis terhadap penyakit Panama. Penelitian ini menggunakan metode in vitro, in planta, dan mengamati efek fungi endofit pada pisang Cavendish. Tiga fungi endofit terpilih, diidentifikasi sebagai Fusarium sp. NMC278, NMC279, dan NMC293, menunjukkan potensi pengendalian biologis yang efektif terhadap penyakit Panama, dengan persentase inhibisi berkisar 10%-11% secara in vitro. Mekanisme pengendalian biologis melibatkan antibiosis melalui pelepasan metabolit sekunder yang mampu menghambat patogen, menunjukkan potensi besar dalam melawan penyakit Panama. Uji in planta menunjukkan pertumbuhan yang diinduksi pada tanaman pisang, tercermin dalam peningkatan diameter batang dan jumlah akar. Deteksi senyawa fenolik menunjukkan bahwa fungi endofit yang diinokulasi berkontribusi pada ketahanan tanaman, dengan reaksi positif terhadap glikosida, tanin, dan saponin. Implikasi edukatif dari penelitian ini merekomendasikan pengembangan ensiklopedia digital (59,7%) sebagai bahan ajar, berdasarkan analisis kebutuhan terhadap guru dan siswa di Sekolah Menengah Atas (SMA).

Uncontrolled Keywords:

biocontrol, endophytic, panama disease, wild banana biokontrol, endofit, panama disease, pisang liar

Subjects:

L Education > L Education (General)
Q Science > Q Science (General)
Q Science > QR Microbiology
S Agriculture > SB Plant culture

Divisions:

02-Fakultas Keguruan dan Ilmu Pendidikan > 84205-Jurusan Pendidikan Biologi
02-Fakultas Keguruan dan Ilmu Pendidikan

Depositing User:

Nn Zahro Fathimah

Date Deposited:

29 Apr 2024 11:52

Last Modified:

29 Apr 2024 11:52

URI:

http://eprints.untirta.ac.id/id/eprint/34502

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