Hidayatul Mustafid, Afif (2023) Ekstrak Kulit Batang Kesambi dan Rumput Laut Sebagai Antibakteri Menggunakan Teknologi Enkapsulasi Berbasis Nanopartikel. Master thesis, Universitas Sultan Ageng Tirtayasa.
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Abstract
Encapsulation is the process of coating a material using another material. The encapsulated material is usually referred to as the core material, active ingredient, internal phase, or filler. Nanoparticles are particles measuring 1-100 nanometers. Nanoparticles aim to overcome the solubility of active substances that are difficult to dissolve, improve poor bioavailability, modify drug delivery systems, increase the stability of active substances and improve absorption. Kesambi plants have antibacterial activity against Escherichia coli, Serratia mercesten, Bacillus subtillis and Micrococcus liteus bacteria. In addition to having antibacterial activity, kesambi plants also have antioxidant, anticancer and kidney stone decay activities. Carrageenan is a hydrocolloid compound derived from the seaweed Rhodophyceae, the type of seaweed used is Gracilaria sp. Carrageenan is also another ingredient used as a substitute for gelatin in capsule shells. This research is expected to be able to provide information regarding the utilization of kesambi and seaweed plants from Cilegon city as antibacterial encapsulation products against Escherichia coli bacteria. The method used is a laboratory method using a comparison method between kesambi extract and seaweed carrageenan with a ratio of 1:4, 2:4, 3:4, and 4:4 based on then testing the antibacterial activity of Escerichia coli and characterizing the uniformity of weight, time crushed, swelling test and Scanning Electron Microscope (SEM) Test. The results of the research that has been done, characterization of pectin, test of antibacterial activity of escerichia coli, characterization of weight uniformity, disintegration time and swelling test. Pectin characterization obtained pectin weight of 87 grams, moisture content of 4%, ash content of 1%, methoxyl content of 9.9% and equivalent weight of 617.3 mg. Escerichia coli antibacterial activity test with a ratio of 1:4, 2:4, 3:4, and 4:4 showed optimum conditions at a ratio of 3:4 with an inhibition zone value of 0.45 mm. The results of the characterization of the capsule shells F1, F2, F3 and F4 obtained good results in the capsule shell weight test and time test. According to the Indonesian Pharmacopoeia V edition, the relative standard deviation of the shell weight test must be <2% and the specifications for the capsule disintegration test time are no more than 30 minutes. The results of the degree of swelling test showed the best results in F4 which was 586.7% because F4 had the largest degree of swelling compared to F1, F2 and F3.
| Item Type: | Thesis (Master) | |||||||||
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| Additional Information: | Enkapsulasi adalah proses pelapisan suatu bahan menggunakan bahan lainnya. Bahan yang dienkapsulasi biasanya disebut sebagai bahan inti, bahan aktif, fase internal, ataupun pengisi. Nanopartikel adalah partikel berukuran 1-100 nanometer. Nanopartikel bertujuan untuk mengatasi kelarutan zat aktif yang sukar larut, memperbaiki bioavailabilitas yang buruk, memodifikasi sistem penghantaran obat, meningkatkan stabilitas zat aktif dan memperbaiki absorbsi.Tumbuhan kesambi memiliki aktivitas antibakteri terhadap bakteri Escherichia coli, Serratia mercesten, Bacillus subtillis dan Micrococcus liteus. Selain memiliki aktivitas antibakteri tumbuhan kesambi juga memiliki aktivitas antioksidan, antikanker dan peluruhan batu ginjal. Karagenan merupakan senyawa hidrokoloid yang berasal dari rumput laut rhodophyceae, jenis rumput laut yang digunakan yaitu gracilaria sp. Karagenan juga menjadi bahan lain yang digunakan sebagai pengganti gelatin pada cangkang kapsul. Penelitian ini diharapkan mampu memberikan informasi mengenai pemanfaatan tumbuhan kesambi dan rumput laut asal kota Cilegon sebagai produk enkapsulasi antibakteri terhadap bakteri Escherichia coli. Metode yang di gunakan yaitu metode laboratorium dengan menggunakan metode perbandingan antara ekstrak kesambi dengan karagenan rumput laut degan perbandingan 1:4, 2:4, 3:4, dan 4:4 yang berbasis kemudian diuji aktivitas antibakteri Escerichia coli dan karakterisasi keseragaman bobot, waktu hancur, uji swelling dan Uji Scanning Electron Microscope (SEM). Hasil penelitian yang telah dilakukan, karakterisasi pektin , uji aktivitas antibakteri escerichia coli, karakterisasi keseragaman bobot, waktu hancur dan uji swelling. Karakterisasi pektin diperoleh bobot pektin sebesar 87 gram, kadar air sebesar 4%, kadar abu sebesar 1% , kadar metoksil sebesar 9,9% dan bobot ekivalen yaitu 617,3 mg. Uji aktivitas antibakteri Escerichia coli degan perbandingan 1:4, 2:4, 3:4, dan 4:4 menunjukan kondisi optimum pada perbandingan 3:4 dengan nilai zona hambat 0,45 mm. Hasil karakterisasi cangkang kapsul F1, F2, F3 dan F4 diperoleh hasil baik pada uji bobot cangkang kapsul dan uji waktu. Sesuai Farmakope Indonesia edisi V bahwa relatif standar deviasi uji bobot cangkang harus < 2% dan spesifikasi syarat waktu uji hancur kapsul adalah tidak lebih dari 30 menit. Hasil derajat uji swelling menunjukan hasil terbaik yaitu pada F4 yaitu 586,7% dikarenakan F4 memiliki derajat swelling terbesar dibandingkan F1, F2 dan F3. | |||||||||
| Uncontrolled Keywords: | kesambi plant, carrageenan, encapsulation, nanoparticles tumbuhan kesambi, karagenan, enkapsulasi, nanopartikel | |||||||||
| Subjects: | T Technology > TP Chemical technology | |||||||||
| Divisions: | 08-Pascasarjana | |||||||||
| Depositing User: | Afif Hidayatul Mustafid | |||||||||
| Date Deposited: | 23 Oct 2023 09:59 | |||||||||
| Last Modified: | 23 Oct 2023 09:59 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/30383 |
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