Rahmayetty, Rahmayetty and Kanani, Nufus and Fauziah, Intan and Ukhdiya, Nurul (2019) Artikel_Pengaruh Laju Pembebanan Substrat terhadap Produksi Asam Laktat Berbahan Baku Molase. Jurnal Integrasi Proses, 8 (2). pp. 76-81. ISSN 2302-9048

Text 5. PENGARUH LAJU PEMBEBANAN SUBSTRAT TERHADAP PRODUKSI ASAM LAKTAT.pdf - Published Version Download (986kB)

Abstract

Lactic acid is a raw material for the biodegradable and biocompatible PLA (polylactic acid) polymer industry. PLA prices are more expensive than conventional plastic. Efforts continue to be made to reduce the cost of PLA production in order to compete with conventional plastics, one of which is to find alternative raw materials that are inexpensive. A promising raw material is molasses. The purpose of this study is to obtain the rate of loading of molasses substrate which produces optimum lactic acid. This research was conducted in stages, namely inoculation of Lactobacillus acidophilus, fermentation of molasses and purification of lactic acid. The bioreactor used in this study was an anaerobic biorector with a volume of 1000 ml. Lactobacillus acidophilus inoculation was carried out in MRS medium at 38°C for 12 hours. In fed batch system the substrate was put simultaneously, with variations in the substrate loading flow rate of 22.2; 33.3; 44.4 and 66.7 ml/hr. The temperature of the fermenter was kept constant at 38oC for 72 hours. The purification process was carried out by adding calcium hydroxide (Ca (OH)2) and acidifying with 0.01 M sulfuric acid solution at a temperature of 70°C to produce lactic acid and calcium sulfate (gypsum). Gypsum and lactic acid were filtered so that lactic acid was separated from gypsum. The highest specific rate of product formation (qp) is 16.065 gP/gS with lactic acid concentration is 8,3 g/L at substrate loading rate of 33.3 m/hr.

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