Nurfadilah, Marwah (2023) ANALISIS KERAGAMAN MORFOLOGI DAN GENETIK TANAMAN JAHE (Zingiber officinale Rosc.) LOKAL DI KABUPATEN LEBAK PROVINSI BANTEN. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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Abstract
This study aimed to determine the morphological diversity, genetic diversity, and kinship relationships of local ginger in Lebak Regency based on morphological characteristics and ISSR molecular markers. This research was conducted in Lebak Regency, Laboratory of Physiology and Biotechnology of the Faculty of Agriculture, University of Sultan Ageng Tirtayasa and the building of Genomics BRIN Cibinong from July 2021 to August 2022. This research used qualitative and quantitative descriptive methods. Morphological characteristic analysis used seven local ginger accessions and molecular analysis used modified Doyle & Doyle (1987) method and three ISSR primers. Morphological kinship data (dissimilarity matrix) were processed in the IBM SPSS statistics Version 26 program, DNA band sizes were processed in the Gel Analyzer 2010 program and grouping analysis using the UPGMA method in the NTSYS Version 2.10e program. The result showed that for the qualitative characters there were seven narrow morphological characters and three broad morphological characters while for the quantitative characters there were two narrow morphological characters and seven broad morphological characters. The morphological analysis showed that the coefficient value of kinship relationships (dissimilarity matrix) ranged from 6.08-27.61. The molecular analysis showed that the coefficient value of genetic similarity ranged from 0.88-1.00 and on dendogram formed three groups so that it was known that there was narrow genetic diversity. It was so clear that morphological and genetic diversity among accessions showed no difference.
| Item Type: | Thesis (S1) | |||||||||
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| Additional Information: | Penelitian ini bertujuan untuk mengetahui keragaman morfologi, keragaman genetik, dan hubungan kekerabatan jahe lokal di Kabupaten Lebak berdasarkan karakteristik morfologi dan penanda molekuler ISSR. Penelitian ini dilaksanakan di Kabupaten Lebak, Laboratorium Fisiologi dan Bioteknologi, Fakultas Pertanian, Universitas Sultan Ageng Tirtayasa dan di Gedung Genomik BRIN Cibinong pada bulan Juli 2021 hingga Agustus 2022. Penelitian ini menggunakan metode deskriptif kualitatif dan kuantitatif. Analisis karakteristik morfologi menggunakan tujuh aksesi jahe lokal dan analisis molekuler menggunakan metode Doyle & Doyle (1987) yang dimodifikasi dan tiga primer ISSR. Data kekerabatan secara morfologi diolah pada program statistik IBM SPSS Versi 26, ukuran pita DNA diolah pada program Gel Analyzer 2010 dan analisis pengelompokan menggunakan metode UPGMA pada program NTSYS. Hasil penelitian menunjukkan bahwa untuk karakter kualitatif terdapat tujuh karakter morfologi sempit dan tiga karakter morfologi luas sedangkan untuk karakter kuantitatif terdapat dua karakter morfologi sempit dan tujuh karakter morfologi luas . Analisis morfologi menunjukkan bahwa nilai koefisien hubungan kekerabatan (dissimilarity matrix) berkisar antara 6,08-27,61. Analisis molekuler menunjukkan bahwa nilai koefisien kemiripan genetik berkisar antara 0,88-1,00 dan pada dendogram membentuk tiga kelompok sehingga diketahui terdapat keragaman genetik yang sempit. Sangat jelas bahwa keragaman morfologi dan genetik di antara aksesi tidak menunjukkan perbedaan. | |||||||||
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
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| Divisions: | 04-Fakultas Pertanian 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi |
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| Depositing User: | Mrs Marwah Nurfadilah | |||||||||
| Date Deposited: | 03 May 2023 10:28 | |||||||||
| Last Modified: | 03 May 2023 10:28 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/23700 |
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