NOVIEN ULINA SILALAHI, HANNA (2022) RESPON EMPAT VARIETAS KEDELAI (Glycine max L.) DAN PEMBERIAN DUA JENIS KOMBINASI MEDIA PADA PEMBENTUKAN KALUS EMBRIOGENIK SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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Abstract
This study aims to determine the response of four soybean varieties (Glycine max L.) and the provision of two types media combination on embryogenic callus formation in vitro. This research was conducted at the Tissue Culture Laboratory, National Research and Innovation Agency from June to September 2019. The research method was used Factorial Randomized Block Design consisting of 2 factors. The first factor is soybean varieties treatment with three levels, which is v0 (Grobogan), v1 (Mutiara 1), v2 (Rajabasa), and v3 (Mitani). The second factor is the two types media combination treatment with four levels, which is m1 (MS + 2,4-D 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm) and m2 (MS + BAP 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm). The results showed that Mitani varieties give the better yield on the percentage of live explants (9.78%) and the percentage of explants forming shoots (9.23%). Grobogan varieties give the better yield on percentage of explans forming embryogenic callus (8.78%) and the percentage of explants forming roots (7.90%). The type of media combination m2 (MS + BAP 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm) gave the better yield on the percentage of live explants (9.78%) and the percentage of explants forming shoots (9.68%). There is an interaction between soybean varieties with the type of media combination on the percentage of live explants and the percentage of explants forming shoots. Keywords : embryogenic callus, in vitro, media combination, soybean varieties
| Item Type: | Thesis (S1) | ||||||||||||
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| Additional Information: | Penelitian ini bertujuan untuk mengetahui respon empat varietas kedelai (Glycine max L.) dan pemberian dua jenis kombinasi media pada pembentukan kalus embriogenik secara in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan, Badan Riset dan Inovasi Nasional pada bulan Juni 2019 hingga Spetember 2019. Rancangan penelitian yang digunakan adalah rancangan acak kelompok (RAK) dengan dua faktor yaitu varietas kedelai dan dua jenis kombinasi media. Faktor pertama adalah varietas kedelai yaitu v0 (Grobogan), v1 (Mutiara 1), v2 (Rajabasaa), dan v3 (Mitani). Factor kedua adalah dua jenis kombinasi media yaitu m1 (MS + 2,4-D 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm) dan m2 (MS + BAP 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm). Hasil penelitian menunjukkan bahwa varietas Mitani memberikan hasil hasil yang lebih baik pada persentase eksplan hidup (9,78 %), dan persentase eksplan membentuk tunas (9,52 varietas Grobogan memberikan hasil yang lebih baik pada persentase eksplan membentuk kalus embriogenik (8,78 %) dan persentase eksplan membentuk akar (7,90 %). Jenis kombinasi media m2 (MS + BAP 0,5 ppm + TDZ 0,5 ppm + NAA 0,5 ppm) memberikan hasil yang lebih baik pada persentase eksplan hidup (9,78 %) dan persentase eksplan membentuk akar (9,68 %). Terdapat interaksi antara varietas kedelai dengan jenis kombinasi media pada persentase eksplan hidup dan persentase eksplan membentuk tunas. Kata kunci : kalus embriogenik, varietas kedelai, kombinasi media, in vitro | ||||||||||||
| Uncontrolled Keywords: | Keywords : embryogenic callus, in vitro, media combination, soybean varieties | ||||||||||||
| Subjects: | S Agriculture > SB Plant culture | ||||||||||||
| Divisions: | 04-Fakultas Pertanian 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi |
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| Depositing User: | Ms Hanna Novien Ulina Silalahi | ||||||||||||
| Date Deposited: | 25 May 2023 10:19 | ||||||||||||
| Last Modified: | 25 May 2023 10:19 | ||||||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/19200 |
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