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UJI ANTAGONIS Pseudomonas sp. ASAL ENDOFIT PERAKARAN PADI TERHADAP PENYAKIT BLAS (Pyricularia oryzae) SECARA IN VITRO

HERDIANI, LINDA (2015) UJI ANTAGONIS Pseudomonas sp. ASAL ENDOFIT PERAKARAN PADI TERHADAP PENYAKIT BLAS (Pyricularia oryzae) SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui bakteri antagonis Pseudomonas sp. endofit akar padi terhadap penyakit blas (Pycularia oryzae) secara in vitro. Penelitian ini dilakukan di Laboratorium Agroekologi, Fakultas Pertanian Universitas Sultan Ageng Tirtayasa pada bulan April hingga Juni 2015. Penelitian ini menerapkan eksperimen satu faktor yang disusun dalam Rancangan Acak Lengkap dengan tiga ulangan. Perawatan terdiri dari 9 isolat Pseudomonas spp. yang disaring dari akar endofit tanaman padi. Hasil pengujian menunjukkan bahwa pengujian in vitro antagonis dapat menghambat pertumbuhan P. oryzae. Penghambatan 9 isolat terhadap pertumbuhan Pseudomonas sp. memiliki zona bening yang signifikan. Isolat Ps 17 (58,89%), Ps 22 (54,44%), Ps 37 (52,06%) dan Ps 39 (61,42%) memiliki daya hambat terbesar. Hasilnya menunjukkan bahwa bakteri Pseudomonas spp. pada penelitian ini berpotensi dalam pengendalian penyakit ledakan di tanaman padi.

Item Type: Thesis (S1)
Contributors:
ContributionContributorsNIP/NIM
Thesis advisorSaylendra, Andree197904202008011013
Thesis advisorBantiar, Tubagus198109202005011004
Additional Information: The purpose of this research was to determine the antagonistic bacteria Pseudomonas spp. of paddy roots endophytic against disease blast (Pycularia oryzae) in vitro. This research was conducted at the Laboratory of Agroecology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa on April to June 2015. This research implemented experimental one factor that arranged in Completely Randomized Design with three replications. The treatment consisted of 9 isolates of Pseudomonas spp. which was screened from endophytic roots of paddy plants. The test results showed that antagonistic in vitro testing could inhibit the growth P. oryzae. Inhibition of 9 isolates to the growth Pseudomonas spp. had the significant clear zone. Isolate Ps 17 (58.89%), Ps 22 (54.44%), Ps 37 (52.06%) and Ps 39 (61.42%) have the largest inhibition. The results showed that the bacteria Pseudomonas spp. on this research is potential on controlling disease blast in paddy plant.
Uncontrolled Keywords: Kata kunci: Pseudomonas spp., Pyricularia oryzae, in vitro, skrining
Subjects: S Agriculture > S Agriculture (General)
Divisions: 04-Fakultas Pertanian
04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi
Depositing User: Perpustakaan Pusat
Date Deposited: 22 Apr 2022 10:47
Last Modified: 07 Sep 2022 09:57
URI: http://eprints.untirta.ac.id/id/eprint/13241

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