AHDIANI, PINA (2015) POTENSI Pseudomonas sp. UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI (Xanthomonas oryzae pv. oryzae) SECARA IN VITRO. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA.
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POTENSI Pseudomonas sp. UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI (Xanthomonas oryzae pv. oryzae) SECARA IN VITRO full.pdf - Published Version Restricted to Registered users only Download (1MB) |
Abstract
Pseudomonas sp. telah diteliti berkaitan dengan kemampuannya dalam mengendalikan berbagai penyakit tanaman. Penelitian ini bertujuan untuk mengetahui potensi Pseudomonas sp. dalam pengendalian penyakit hawar daun bakteri (Xanthomonas oryzae pv. oryzae) di vitro. Isolat bakteri yang digunakan adalah eksplorasi tanaman padi. Xanthomonas oryzae pv. oryzae diisolasi dari daun tanaman padi yang terserang bakteri dari Desa Terumbu, Kecamatan Kasemen, Kabupaten Serang dan Pseudomonas sp.was diisolasi dari tanah dan akar tanaman padi dari daerah Unyur di kabupaten Serang, Kediaman Serang. Berdasarkan hasil penelitian ditemukan 54 Pseudomonas sp. isolat bakteri yang berhasil diisolasi. Ada 6 terbaik isolat (Ps 6, Ps 29, Ps 39, Ps 40, Ps 45, dan Ps 46) dari skrining pertama yang diyakini sebagai bakteri antagonis terhadap Xanthomonas oryzae pv. oryzae bakteri. Isolat dipilih untuk dilakukan penyaringan ulang dan juga untuk menentukan yang terbaik penghambatan masing-masing isolat. Penghambatan Pseudomonas sp. isolat bakteri adalah diuji dan menunjukkan bahwa hasilnya tidak berbeda nyata antara masing-masing isolat maupun terhadap kontrol (tanpa perlakuan bakteri). Ps 39 isolat cenderung memiliki zona bening yang lebih tinggi dibandingkan dengan isolat lainnya. Pseudomonas sp. Bakteri memiliki morfologi koloni yang bervariasi pada cawan petri (piring agar) dan agar miring. Pseudomonas sp. bakteri cenderung non patogen, tetapi ada beberapa Pseudomonas sp. bakteri menjadi patogen, dan Pseudomonas sp. bakteri cenderung menjadi gram negatif.
| Item Type: | Thesis (S1) | |||||||||
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| Additional Information: | Pseudomonas sp. have been research relates to its ability in controlling various plant diseases. This research was aimed to determine potential of Pseudomonas sp. in controlling bacterial leaf blight disease (Xanthomonas oryzae pv. oryzae) in vitro. Bacterial isolates used an exploration of rice plant. Xanthomonas oryzae pv. oryzae was isolated from the leaves of rice plant that attacked by bacteria from Terumbu Village, Kasemen District, Serang Residence and Pseudomonas sp.was isolated from soil and roots of rice plant from Unyur area in Serang district, Serang Residence. Based on result of the research, there were 54 Pseudomonas sp. bacteria isolates which have been succeed to be isolated. There were 6 best isolates (Ps 6, Ps 29, Ps 39, Ps 40, Ps 45, dan Ps 46) from first screening which was believed to be bacterial antagonist against Xanthomonas oryzae pv. oryzae bacteria. Isolates were chosen to do re-screening and also to determine the best inhibition of each isolate. The inhibition of Pseudomonas sp. bacteria isolates was tested and showed that the results were not significantly different neither it was between each isolates nor against control (without treatment bacteria). Ps 39 isolate tend to had higher clear zone compare to other isolates. Pseudomonas sp. bacteria had various colony morphology on petridish (agar plate) and agar slant. Pseudomonas sp. bacteria tend to be non pathogen, but there were some of Pseudomonas sp. bacteria to be pathogen, and Pseudomonas sp. bacteria tend to be negative gram. | |||||||||
| Uncontrolled Keywords: | Kata kunci: Pseudomonas sp., Xanthomonas oryzae pv. oryzae, in vitro, skrining, antagonis. | |||||||||
| Subjects: | S Agriculture > S Agriculture (General) | |||||||||
| Divisions: | 04-Fakultas Pertanian 04-Fakultas Pertanian > 54211-Program Studi Agroekoteknologi |
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| Depositing User: | Perpustakaan Pusat | |||||||||
| Date Deposited: | 21 Apr 2022 14:51 | |||||||||
| Last Modified: | 23 Aug 2022 10:50 | |||||||||
| URI: | http://eprints.untirta.ac.id/id/eprint/12800 |
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